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The dependence of the osmotic resistance of rat erythrocytes and the concentration of thiol groups of proteins to their membrane from the duration of moderate hypothermia

Guseynov German Omarovich  (associate Professor, Department of General and biological chemistry Of the "Dagestan state medical Academy" of Ministry of health of the Russian Federation )

In the article, a study, moderate hypothermia of different duration effect on the osmotic fragility of erythrocytes and also investigated the content of SH-groups in total protein and membrane protein band 3 of erythrocytes of rats. For experiments carried out the following: body temperature of rats for 30 min was reduced to 30°C by external cooling, and then prolonged hypothermia for 90 and 180 min. Analysis of the data shows that in the dynamics of hypothermia observed reversible changes in osmotic fragility of erythrocytes and the content of thiol groups in proteins membranes. Immediately after the reduction of body temperature and after 90 min of hypothermia osmotic fragility of erythrocytes is increased and after 180 min hypothermia is returned to the control level. The content of SH-groups in the total proteins of erythrocyte membranes and in the protein band 3 after 90 min hypothermia decreased significantly (by 16.4% and 28.8%, respectively), and after 180 min hypothermia is increased to control level. In the dynamics of hypothermia found a correlation (r = -0.950) between the change in osmotic fragility of erythrocytes and the level of thiol groups in proteins membranes. The obtained data show that from homeothermic organisms with prolonged moderate hypothermia may include adaptive mechanisms of the rearrangements of metabolic processes at the level of red blood cells.

Keywords:protein band 3, hypothermia, rats, osmotic hemolysis, thiol groups, red blood cells.

 

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Citation link:
Guseynov G. O. The dependence of the osmotic resistance of rat erythrocytes and the concentration of thiol groups of proteins to their membrane from the duration of moderate hypothermia // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2017. -№06. -С. 85-89
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