Influence of zeolite-containing diatomaceous earth on the mineral composition of saliva

Malov I. V., Ivanov L. N., Kolotilova M. L.

The purpose of this article is to assess the effect of zeolite-containing Tripoli on the mineral composition of saliva Materials and methods. Experiments were carried out on male Wistar rats weighing about 200-250 grams. In total, four groups of experimental rats took part in the experiment. The first group (control) included 26 rats, and this group was intact in all directions. The second group (n=26) included animals that together with the basic feed received zeolite-containing Tripoli in the amount of 1.5 g per kilogram of body weight in addition to the standard diet. The third group (n=26) was on a cariesogenic diet, and the fourth group (n=26) – on a cariesogenic diet, however, rats of this group, along with the base feed, were also additionally given a zeolite-containing Tripoli in the amount of 1.5 g per kilogram of body weight. The duration of the experiment in all experimental groups was 60 days. On the 61st day, the number and depth of carious lesions of the teeth and the degree of atrophy of the alveolar process of the mandible were calculated, the biological material was subjected to laboratory, biochemical and morphological studies. Laboratory tests were performed using automated hematological, immunochemical and biochemical analyzers (Abbott, USA) using original reagents and standardized methods with mandatory weekly controls and monthly calibrators. Automated statistical processing of the results was carried out using the program Statistica-6, as well as, in order to duplicate and calculate parallel values, using the current package of applications Excel for Windows. The critical level of the reliable significance of the analyzed statistical hypotheses in the conducted dissertation research was taken as less than 0.05. Results. Analyzing the results of the study of calcium, phosphorus and magnesium in the study groups, it should be noted first of all a significant decrease in these indicators in animals on a karyosogenic diet (III study group), which indicates a deficiency of these elements. At the same time, the study of indicators in the group of animals on a karyosogenic diet allows to testify to the effectiveness of zeolite-containing diatomaceous earth, with respect to the restoration of the level of these trace elements, as evidenced by statistically significant differences in indicators in the III and IV study groups. Conclusion. Cariesogenic diet helps to reduce calcium, phosphorus and magnesium in the oral fluid. Zeolite diatomaceous earth helps to restore the mineral composition of the oral fluid, it is very important anticarcinogen factor.

Keywords:zeolite-containing Tripoli, cariesogenic diet, calcium, phosphorus, magnesium