Dumchenko Natalya Borisovna (Researcher of Federal Budgetary Research Institution "State Research Centre of Virology and Biotechnology "Vector" of the Federal Service for Surveillance in Consumer Rights Protection and Human Well-being, Koltsovo, Novosibirsk Region, Russian Federation)
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Nutrient medium and serum of animals are necessary components for cell cultivation. Nutrient medium has a certain chemical composition. Whey, as a product of animal origin, remains an unexplored complex of various components, creates non-standard conditions for cell growth and reproduction. In this paper, the effect of various hydrolysates as a serum substitute is studied. The stimulating effect of HyPep 2209 soybean hydrolyzate on the viability of the MDCK cell culture when it is added to the basic nutrient medium in various concentrations is shown. The optimal concentration of the hydrolyzate HyPep 2209 for the cultivation of transplantable MDCK lines has been determined.
Keywords:serum-free nutrient medium, cell culture, hydrolysates
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Citation link:
Dumchenko N. B. Study of the effect of plant hydrolysates on the viability of a culture of MDCK cells // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2018. -№12/2. -С. 9-12 |
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