Evaluation of the infl uence of point spread function of the recording instrument on the results of analytical spots scanning in the microplate wells using the FOSPHAN technology

Darashkevich Y., Asliyan S., Sokolov A.

A method is proposed for determining the distorting function (PSF) of a scanning device using the FOSPHAN technology. A quality criterion for modeling PSF is proposed. Studies have been conducted using a control sample with the aim of further adjusting the PSF based on the proposed criterion. The optimal values of the two-dimensional normal distribution, simulating a PSF, are determined. Conducted research on real samples to determine the effect of the PSF recording device on the scan results. The degree of distortion of the initial values for different levels of the intensity of the recorded signal is estimated. An improvement in the signal-to-noise ratio in the reconstructed image is shown.

Keywords:Time-resolved luminescence, multiplex analysis, digital image, deconvolution, signal-to-noise ratio