Assessment of the content of HSC and MMSC in the bone marrow of tubular bones of the lower extremities after amputation

Nikolaeva L. P.

Introduction: New high-tech medical advances offer prospects for treatment of many diseases. New treatments are achieve a more pronounced therapeutic effect. The introduction of cellular technology, the most advanced of them. Materials and methods: The study included 40 patients with different diseases and different age. These patients underwent lower limb amputation if unable to save it. It was patients with gangrene of the lower limb as a result of ischemia, traumatic injuries, road accidents, hypothermia. Patients were studied in the bone marrow immediately after amputation of the lower extremity. In the laboratory determined the number of hematopoietic and mesenchymal stem cells using specific markers. All samples were carried out the immunohistochemically characterization of the bone marrow. Results: The results show that the bone marrow of different patients has a certain characteristic with constant parameters. Data show the biological value of bone marrow removed with amputation of the lower limb of the patient. The ease of obtaining bone marrow makes the procedure in surgical practice. The high content of hematopoietic and mesenchymal stem cells shows the biological value of bone marrow bones. This gives the opportunity to their use in autologous cell therapy for the patient.

Keywords:amputation of a limb, bone marrow, autologous cell therapy, stem cells