Usoltseva P. S. (post-graduate student, FSBEI HE «Urals State Medical University» of the Ministry of Healthcare of Russian Federation (Ekaterinburg); researcher, Ekaterinburg Research Institute of Viral Infections FBRI SRC VB «Vector», Rospotrebnadzor)
Novoselov A. V. (Candidate of Medical Sciences, Laboratory Specialist, LLC Medical-Pharmaceutical Center «Garmonia» (Ekaterinburg))
Rezaykin A. V. (Candidate of Medical Sciences, Ekaterinburg Research Institute of Viral Infections FBRI SRC VB «Vector», Rospotrebnadzor)
Sergeev A. G. (Doctor of Medical Sciences, FSBEI HE «Urals State Medical University» of the Ministry of Healthcare of Russian Federation (Ekaterinburg))
Alimov A. V. (Candidate of Medical Sciences, Ekaterinburg Research Institute of Viral Infections FBRI SRC VB «Vector», Rospotrebnadzor)
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The article presents a comparative study of the uncoating kinetics for the echovirus 11 variants that use different binding receptors (DAF or FcRn) and endocytic pathways (Arf6-associated or clathrin-dependent) to enter human rhabdomyosarcoma (RD) cells. Various effects of nocodazole were revealed by comparing kinetics of the infectious activity in the single cycle growth experiments with echovirus 11 variants. An increased concentration of FcRn in the perinuclear area of the cells was observed by immunofluorescence. A hypothesis of the intracellular convergence for the traffic of the echovirus 11 complex with the binding receptor DAF and the uncoating receptor FcRn in the perinuclear endocytic recycling compartment is discussed.
Keywords:uncoating, echovirus, cell receptors, DAF, FcRn
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Citation link:
Usoltseva P. S., Novoselov A. V., Rezaykin A. V., Sergeev A. G., Alimov A. V. Effects of nocodazole on the kinetics of echovirus 11 variants uncoating after Arf6-associated or clathrin-dependent internalization in RD cells // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2020. -№03/2. -С. 36-43 |
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