Features of development of mono- and polycultures of bacteria on glass with different relief

Korlyakov K. A.

We studied the population of Staphylococcus aureus and Pseudomonas aeruginosa cultures and their joint polyculture of glasses with different recess diameters: 7.8 microns, 23.4 microns, 78 microns, and 235 microns. It was found that the larger the diameter of the furrows, the faster they form a monolayer of bacteria, as the previous stage of biofilm development. Individual preferences of crops for settlement of certain size depressions of sectors are revealed. The number of bacterial cells in the recesses is always higher than between the recesses, which may indicate a more intensive accumulation of nutrient medium inside the recesses. At the same time, the number of cells in the recesses at the first hours of exposure (5-7 hours) is higher than in the control, and in the following hours, the number in the control on the contrary begins to prevail over the other sectors. This can be explained by the fact that uneven depressions on the glass are a catalyst for the development of bacteria due to the accumulation of nutrients there and a place convenient for attaching cells. In the future, when forming a biofilm, the surface relief no longer plays a role in the development of culture. In relation to polyculture, similar trends were revealed when the Pseudomonas aeruginosa culture replaced the Staphylococcus aureus culture.

Keywords:"scratched" glass, monolayer, cell count, sector, Staphylococcus aureus, Pseudomonas aeruginosa.