Influence of biomass quantity on the formation of silver nanoparticles by saccharomyces ellipsoideus BSU-XR1

Azadaliyeva Saadat Fazaim, Ganbarov Khudaverdi Ganbar, Jafarov Mirmusa Mirish, Huseynova Sanam Ismail

The aim of current study was to investigate the ability of silver nanoparticles formation by Saccharomyces ellipsoideus BSU-XR1 depending on the biomass quantity. As a result of UV – VIS spectrophotometric analyses of the obtained reaction mixture, it has been determined that the high amount of nanoparticles was observed in range of 5-15g wet biomass. Yield of nanoparticles in 20 g biomass was very low. When specimens taken from each mixture were examined on a scanning electron microscope, the size of silver nanoparticles were found to be 8.63 – 16.9 nm in 5 g biomass, 17.2 nm in 10 g biomass and 22.4 nm in 15 g biomass.The formed silver nanoparticles were round shaped in all three specimens. X-ray spectroscopic analyses of the samples have demonstrated the specific adsorption peak (Ag Lal) for silver nanoparticles. It has been established that when the amount of wet biomass of Saccharomyces ellipsoideus BSU-XR1 increased from 5 to 15 g in the reaction mixture, the size of nanoparticles increased by 1.3-2.6 times. It was concluded that optimal biosynthesis of silver nanoparticles was obtained in10g of biomass.

Keywords:Saccharomyces ellipsoideus, biomass, silver nanoparticles, UV spectrophotometer, scannig electron microscope, X-ray spectroscope.