Screening of bacteria with antibacterial activity against Bacillus subtilis and Escherichia coli strains carrying plasmids that provide resistance to known antibiotics

Aghayeva Aytan Gabil, Huseynova Irada Mammad, Streatfield StephenJohn

The main goal of the presented work was to exclude from the list of "isolates of interest" isolated from oil-contaminated soils of Azerbaijan and possessing antibacterial activity in the supernatant, strains producing well-known and commercially available antibiotics. To achieve this goal, the selected bacterial strains were screened against Bacillus subtilis and Escherichia coli strains carrying plasmids that provide resistance to certain antibiotics, such as erythromycin, kanamycin, chloramphenicol, lincomycin, spectinomycin, tetracycline, ampicillin and streptomycin. As a result of the carried-out experiments, all 14 bacterial strains of interest overcome the resistance mechanisms present in the indicator strains.

Keywords:Antibacterial agents, antimicrobial activity, antibiotics, bioactive molecules, natural products, pathogenic bacteria, minimum inhibitory concentration