Search for nonribosomal peptidesynthetase genes in cultures of soil bacilli-as a way to predict the biofungicidal activity of strains

Vasilchenko Nikita G.

The article provides the results on the study of the nonribosomal peptidesynthetases genes spectrum in aerobic spore-forming bacteria of the genera Bacillus and Paenibacillus. The gene spectrum of nonribosomal peptidesynthetases was analyzed in strains with different levels of antagonistic activity against Fusarium fungi, one of the main pathogens of cereal crops. The study revealed that strains with a higher level of antagonism had at least two genes of different operons of nonribosomal peptidesynthetases each, while strains without strong antagonistic properties had only one gene each. The data obtained can be used to develop a method for the rapid search for strains with biofungicidal activity.

Keywords:aerobic spore-forming bacteria, Fusarium, NRPS, biofungicides, antagonism.