STUDY OF GENOTOXICITY OF DIOXIDIN ON CAENORHABDITIS ELEGANS BY GEL ELECTROPHORESIS

Elbek Albertovich Machigov, Dzhambetova Petimat Makhmudovna, Abilev Serikbai Karimovich

Objective: to study the ability of the antibacterial drug dioxidin to induce DNA breaks in C. elegans cells using the method of total gel electrophoresis of nematode genetic material. One of the promising ones for widespread introduction into practice of genotoxicological studies is the nematode Caenorhabditis elegans, which is characterized by simplicity of cultivation and is a model object in various genetic studies. Methods: in the study, a culture of C. elegans Bristol N2 nematodes was used as a test system, which was grown at 21oC on a solid agarose NGM medium on a nutrient lawn, a culture of Escherichia coli OR50 bacteria was used. Dioxidin was used as a genotoxicant, the genotoxic properties of which have been confirmed in many studies with prokaryotes. Positive controls - betapropiolactone (0.015M) and hydrogen peroxide (0.1M), antioxidant - N-acetyl-cysteine (ACC). Results: the study of the genotoxicity of dioxidin on C. elegans by gel electrophoresis of total DNA showed that dioxidin at concentrations of 0.002M – 0.0045M caused breaks in the DNA molecules of the nematode. In turn, the antioxidant N-acetyl-cysteine at concentrations of 0.01M and 0.001M reduced the level of genotoxicity of dioxidin, which reflects the concentration-dependent protective effect of the antioxidant ACC, which reduces the number of DNA breaks caused by dioxidin. Conclusions: the study of the genotoxicity of the antibacterial agent dioxidin on the nematode C. elegans has shown that it is a sensitive test system, and therefore can be recommended for testing chemical environmental factors for genotoxicity.

Keywords:Caenorhabditis elegans, dioxidin, genotoxicity, DNA breaks, oxidative stress, test system, antioxidant acetylcysteine