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THE RESEARCH OF MICROBIOLOGICAL DIVERSITY OF THE AIR OF THE CAVE OF TAVRIDA

Logadyr Tatyana Alekseevna  (Candidate of Medical Sciences, Associate Professor, Institute “Medical Academy named after S.I. Georgievsky" V. I. Vernadsky Crimean Federal University, Simferopol )

Reshetnik Galina Vasilievna  (PhD, Associate Professor, Institute “Medical Academy named after S.I. Georgievsky" V. I. Vernadsky Crimean Federal University )

Sataieva Tatyana Pavlovna  (Doctor of Medical Sciences, Professor, Institute “Medical Academy named after S.I. Georgievsky" V. I. Vernadsky Crimean Federal University, Simferopol )

Sidyakin Andrey Ivanovich  (Candidate of Biological Sciences, Associate Professor, Institute of Biochemical Technologies, Ecology and Pharmacy, V. I. Vernadsky Crimean Federal University, Simferopol )

Samokhin Gennady Viktorovich  (Senior Lecturer of the Faculty of Geography, Institute "Tauride Academy" V. I. Vernadsky Crimean Federal University, Simferopol )

Krutikov Evgeniy Sergeevich  (Doctor of Medical Sciences, Professor, Director of the Institute “Medical Academy named after S.I. Georgievsky" V. I. Vernadsky Crimean Federal University, Simferopol )

Andreev Ilya Igorevich  (5th year student, Institute “Medical Academy named after S.I. Georgievsky" V. I. Vernadsky Crimean Federal University, Simferopol )

For the first time in the Taurida cave, a study was made of the characteristics of microbial communities that form in the conditions of the cave. As part of the study, the total microbial number (TMC) of the air, cultural, morphological and tinctorial properties of isolated bacteria and fungi were studied. Gram-positive bacteria were identified from the colonies grown on nutrient media: rods, cocci, filamentous forms belonging to the putative genera Sarcina, Staphylococcus, Arthrobacter, Corynebacterium, Brevibacterium. There were single colonies of mold fungi of the genera Aspergillum, Penicillium. These microorganisms are typical for the air of the cave and rocky surfaces. In hard-to-reach places where tourists are prohibited, the maximum growth of anaerobes was revealed. The growth of bacteria of the Escherichia coli group was not detected on the Endo medium. Since the Taurida cave was opened in 2018, and the excursion flow is from mid-2022, the anthropogenic pressure on the microbiota is not yet felt. These studies will be continued in order to study the diversity of microorganisms, quantitative composition and species.

Keywords:Taurida cave, anaerobes, total microbial count, washings, nutrient media, research points

 

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Citation link:
Logadyr T. A., Reshetnik G. V., Sataieva T. P., Sidyakin A. I., Samokhin G. V., Krutikov E. S., Andreev I. I. THE RESEARCH OF MICROBIOLOGICAL DIVERSITY OF THE AIR OF THE CAVE OF TAVRIDA // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2023. -№04. -С. 28-33 DOI 10.37882/2223–2966.2023.04.20
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