USE OF ANTIOXIDANTS IN THE IN VITRO CLONING OF BLACK RASPBERRY

Solovykh Natalya Vladimirovna

The aim of the study was to study the effect of exogenous antioxidants on clonal micropropagation of black raspberry (Rubus occidentalis L.). Methods. At different stages of clonal micropropagation, ascorbic acid, reduced glutathione and rutin were added to the nutrient media at concentrations of 0 (control); 0.25; 0.5; 0.75 and 1 mM. At the stage of introduction into culture, the number of viable explants was taken into account, at the stage of multiplication - the multiplication coefficient and the length of shoots, at the rooting stage - the number of rooted plants, the number and length of roots. Results. At the stage of introduction into the culture, the addition of ascorbic acid or reduced glutathione to the nutrient media increases the number of viable explants in the Cumberland variety by 64 - 73.1%, in the yellow-fruited mutant of Cumberland variety - by 72.2 - 100%. At the multiplication stage, these antioxidants made it possible to increase the reproduction rates of black raspberries by an average of 30–35%. Reduced glutathione increases the average shoot length of black raspberry by 19.8 – 33.3%.The optimal concentration of ascorbic acid was 0.75 mM, optimal concentration of reduced glutathione was 0.5 mM. Ascorbic acid and reduced glutathione had no effect on the process of rhizogenesis. The use of rutin in any of the tested concentrations did not demonstrate a stimulating effect on the viability of sterilized explants, the processes of growth, reproduction and rooting of plants in vitro. Conclusions. The efficiency of clonal micropropagation of black raspberry at the stage of introduction into a sterile culture and at the stage of multiplication can be increased by adding ascorbic acid at a concentration of 0.75 mM or reduced glutathione at a concentration of 0.5 mM to the nutrient media.

Keywords:black raspberry, in vitro, clonal micropropagation, ascorbic acid, reduced glutathione.