INFLUENCE OF ACTIVE OXYGEN SPECIES ON STRUCTURAL AND FUNCTIONAL PARAMETERS OF BRAIN LACTATE DEHYDROGENASE

Khalilov Rustam Abdurazakovich, Jafarova Albina Mekhyadinovna, Rabadanova Zukhra Guseynovna, Jafarov Mursal Binievich

The activity and some physicochemical parameters of rat brain lactate dehydrogenase (LDH) were studied in a model chemical system that generates ROS (Fenton's medium). It was found that the activity of LDH in the brain of rats decreases, and the rate of inactivation of the enzyme is nonlinear in time. A study of the intrinsic fluorescence of LDH showed a decrease in the intensity of the total fluorescence of LDH, which may indicate small conformational changes in the structure of the enzyme. At the same time, a study of the content of sulfhydryl and carbonyl groups in the LDH molecule found that a decrease in LDH activity negatively correlates with an increase in the content of carbonyl groups and positively correlates with a decrease in the content of sulfhydryl groups in the LDH preparation.

Keywords:rats, oxidative stress, lactate dehydrogenase, reactive oxygen species.