Sharov Timur Nikolaevich (Ph.D. med. sciences, senior researcher of laboratory of proteomic analysis
Federal Government Health Institution «Volgograd Plague Control Research Institute» of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Wellfare
)
Korol Ekaterina Vasilievna (researcher of laboratory of proteomic analysis
Federal Government Health Institution «Volgograd Plague Control Research Institute» of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Wellfare
)
Budchenko Anatoly Alexandrovich (Ph.D. biol. sciences, senior researcher of laboratory of proteomic analysis
Federal Government Health Institution «Volgograd Plague Control Research Institute» of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Wellfare
)
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The article is devoted to the assessment of differences in the pools of general cellular proteins of the melioidosis pathogen and closely related microorganisms of the genus Burkholderia.
The aim of the work was to search for potential protein markers specific for the species Burkholderia pseudomallei. Methods. The objects of the study were the collection strains of Burkholderia pseudomallei, B. mallei, B. thailandensis, B. cepacia, as well as individual proteins isolated from electropherograms of these strains. The cell mass of the strains grown on a dense nutrient medium was disinfected, then the protein components were separated by two-dimensional electrophoresis in polyacrylamide gel. The spots of interest were cut out, digested with trypsin, and then identified using the MASCOT search engine. Results. About 75 individual proteins were isolated and analyzed. Proteins specific both to all the studied strains of the genus Burkholderia and to strains of the "Burkholderia pseudomallei" group were determined. It has been shown that the two-dimensional electrophoresis method is not effective enough to detect differences in protein pools both among different species of pathogenic Burkholderia and among strains of the same species.
Keywords:melioidosis, glanders, mass spectrometry, proteomic analysis, electrophoresis.
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Citation link:
Sharov T. N., Korol E. V., Budchenko A. A. MASS-SPECTROMETRIC ANALYSIS PF PROTEINS OF DIFFERENT STRAINS OF PATHOGENIC BURKHOLDERIA // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2024. -№12. -С. 40-44 DOI 10.37882/2223-2966.2024.12.42 |
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