MOLECULAR MECHANISMS OF PEPPER RESISTANCE TO BACTERIAL BLACK SPOT

Dubina Elena Viktorovna, Yevtushenko Ilya Dmitrievich

The article is devoted to the study of molecular mechanisms of sweet pepper (Capsicum annuum L.) resistance to bacterial black spot, the causative agent of which is bacteria of the genus Xanthomonas. The paper considers the features of plant defense reactions at the molecular level, including the participation of specific genes and signaling cascades, activation of the immune response and possible accumulation of antimicrobial compounds. The experimental part of the study includes the analysis of 15 pepper varieties using PCR and primers of the 25-1 series aimed at identifying potential resistance loci. The obtained amplification profiles were analyzed using gel electrophoresis methods and bioinformatics resources (KEGG, NCBI), which made it possible to preliminarily differentiate the varieties by the degree of possible resistance. The efficiency of using molecular marker approaches in breeding aimed at creating forms resistant to bacterial pathogens is substantiated. The presented results expand the understanding of the genetic basis of resistance in cultivated plants and can be used in applied genetics and plant growing.

Keywords:molecular mechanisms, resistance, Capsicum annuum L., bacterial black spot, Xanthomonas spp., PCR analysis, primers 25-1, amplification profile, marker-assisted selection, bioinformatics, plant breeding