Korol Ekaterina Vasilievna (Researcher, Proteomic Analysis Laboratory
FKUZ " Volgograd Anti-Plague Research Institute" of Rospotrebnadzor
)
Kuznetsova Tatyana Vladimirovna (Resident of the Laboratory of Rapid Diagnostics of Infectious Diseases
FKUZ " Volgograd Anti-Plague Research Institute" of Rospotrebnadzor
)
Teteryatnikova Natalia Nikolaevna (Resident of the Laboratory of Rapid Diagnostics of Infectious Diseases
FKUZ " Volgograd Anti-Plague Research Institute" of Rospotrebnadzor
)
Sharov Timur Nikolaevich ( Ph.D. med. sciences, senior researcher of laboratory of proteomic analysis
FKUZ " Volgograd Anti-Plague Research Institute" of Rospotrebnadzor
)
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The article is devoted to the evaluation of the efficiency of different methods for isolation and concentration of the whole virion of West Nile fever virus.
The aim of the work was to find the optimal method for obtaining the whole virion antigen of West Nile fever virus. Methods. The objects of the study were collection strains of West Nile fever virus, as well as fractions isolated from the virus-containing fluid. The virus strains cultivated in Vero cells were disinfected, then the cell components and virions were separated by ultracentrifugation, precipitation and chromatography. The analysis of the preparations was carried out using polyacrylamide gel electrophoresis and ELISA. Results. Five samples of material presumably containing the virus were isolated and analyzed. It was determined that a single band in the region of 65 kDa on the electrophoregram most likely corresponds to the whole virion antigen of West Nile fever virus. It was shown that the method of ion-exchange chromatography with the sorbent "CHT Type I" turned out to be optimal for solving the problem, since it allowed the most effective concentration and purification of the whole-virion antigen of WNV.
Keywords:West Nile virus, chromatography, ultracentrifugation, antigen
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Citation link:
Korol E. V., Kuznetsova T. V., Teteryatnikova N. N., Sharov T. N. EVALUATION OF THE EFFICIENCY OF VARIOUS METHODS OF OBTAINING WEST NILE VIRUS WHOLE VIRION ANTIGEN // Современная наука: актуальные проблемы теории и практики. Серия: Естественные и Технические Науки. -2025. -№10. -С. 15-18 DOI 10.37882/2223-2966.2025.10.21 |
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