EXPERIMENTAL SUBSTANTIATION OF THE METHOD OF STIMULATION OF ADHESIONS IN THE PLEURAL CAVITY IN LABORATORY ANIMALS

Khmara Artyom Dmitrievich, Kapralov Sergey Vladimirovich, Polidanov Maxim Andreevich, Bazarov Dmitry Vladimirovich, Volkov Kirill Andreevich, Petrunkin Rodion Pavlovich

The aim of the study is experimental substantiation of the method of stimulation of adhesion process in the pleural cavity in laboratory animals. Materials and methods. The studies were carried out on 80 female laboratory rats of “standard” breed weighing 200±50 g. All experimental animals were divided into two groups: 40 rats in each group. The first group included experimental animals, which underwent pleurodesis, acting only on the parietal sheet of pleura. The second group included experimental animals that underwent pleurodesis of visceral and parietal pleural sheets. General anesthesia of laboratory animals was performed according to the standard technique. Under aseptic conditions a thoracotomy was performed through 5-6 intercostal spaces from periorbital to midclavicular line. Atypical lung resection was performed. Further, the healthy (unchanged) lung area was exposed to the solid-state laser radiation (laser device Lasermed 1-10 (Russia), near-infrared range) with a wavelength of 1064 nm, power 7-8 W (the optimal power of 7-8 W was selected experimentally; up to 6 W hemostasis is not ensured, over 10 W - excessive destruction occurs). Exposure to pleural sheets was carried out with the help of quartz light guide by non-contact method, but not more than 2 cm from pleura and visual assessment of the appearance of signs of coagulation. At direct contact with pleura its destruction occurs, and more than 2 cm - there will be no coagulation, this is due to the power density of the laser beam. Exposure was carried out under visual control and stopped when signs of coagulation appeared. After that the chest wall wound was sutured. Results. Due to this method in the experimental animals of the second group in comparison with the first group there was an earlier, more pronounced and voluminous adhesion process, which was confirmed by the results of histological study. Conclusions. Thus, the morphologic study confirmed that adhesion of visceral and parietal pleural sheets was achieved in the animals of the first group.

Keywords:bullous emphysema, minimally invasive thoracoscopic surgeries, stimulation of adhesion process, laser surgery